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R&D Systems biotinylated goat antihuman il 1ra

Figure 2. LAP fusion proteins are expressed and secreted by 293T cells and can be cleaved with MMP1 to release the free cytokine/ growth factor. Western blotting of (A) recombinant LAP-mmp-BMP-7 detected with anti-BMP-7; (B) recombinant LAP-mmp- IL-10detectedwithanti-IL-10;(C)recombinantLAP-mmp-IL-4detectedwithanti-IL-4;(D)recombinantLAP-mmp-IL-EPOdetectedwith anti-IL-EPO; (F) recombinant LAP-mmp-IL-17 detected with anti-IL-17; (E,G and H) recombinant <t>LAP-mmp-IL-1ra,</t> LAP-mmp-TGF-b and LAP-mmp-IGF1 respectively detected with anti-LAP antibody. All blots indicate via the + or -- sign the presence or absence of MMP1.

Biotinylated Goat Antihuman Il 1ra, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 12 article reviews

biotinylated goat antihuman il 1ra - by Bioz Stars, 2026-08

93/100 stars

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1) Product Images from "Latency can be conferred to a variety of cytokines by fusion with latency-associated peptide from TGF-β."

Article Title: Latency can be conferred to a variety of cytokines by fusion with latency-associated peptide from TGF-β.

Journal: Expert opinion on drug delivery

doi: 10.1517/17425247.2013.839655

Figure Legend Snippet: Figure 2. LAP fusion proteins are expressed and secreted by 293T cells and can be cleaved with MMP1 to release the free cytokine/ growth factor. Western blotting of (A) recombinant LAP-mmp-BMP-7 detected with anti-BMP-7; (B) recombinant LAP-mmp- IL-10detectedwithanti-IL-10;(C)recombinantLAP-mmp-IL-4detectedwithanti-IL-4;(D)recombinantLAP-mmp-IL-EPOdetectedwith anti-IL-EPO; (F) recombinant LAP-mmp-IL-17 detected with anti-IL-17; (E,G and H) recombinant LAP-mmp-IL-1ra, LAP-mmp-TGF-b and LAP-mmp-IGF1 respectively detected with anti-LAP antibody. All blots indicate via the + or -- sign the presence or absence of MMP1.

Techniques Used: Western Blot, Recombinant

Figure 3. LAP fusion proteins are latent until activated by cleavage with MMP. Varying dilutions of supernatant from 293Tcells transiently transfected with (A) LAP-IL-10 (with or without MMP treatment) were added to D36 cells (mast cell line). *P £ 0.05 or (B) LAP-IL-4 (with or without MMP treatment) were added to CT6 cells. The effects of LAP-IL-4 on cell proliferation was measured (n = 3) 72 h later (C) HeLa cells permanently transfected with reporter gene NF-kB-luciferase were treated with IL-1b in the presence of varying concentrations of 293Tsupernatants containing LAP-IL-1ra (SEM values too small to be plotted). (D) Effect of LAP-TGF-b on mink lung fibroblast cells expressing PA-1 promoter driven luciferase. *** p = 0.0001. (E) Supernatants from transiently transfected 293T cells expressing LAP-IGF1 were added to CG4 cells. ** p = 0.001 by paired t-test (F) Proliferation of the megakaryoblastic cell line UT-7 was measured after incubation with supernatants from 293T cells transiently transfected with LAP-EPO. Inhibition of MMP by TAPI-2 prevents background EPO release (left panel) (G) Human fetal fibroblasts cells were stimulated for 24 h with 293T cell-supernatants transiently transfected to express human full-length IL-17A or LAP-IL-17A treated or not with MMP1. * pp = 0.0002, ** p = 0.000001. (H) LAP-TGF-b and LAP BMP-7 promote chondrogenesis in vitro in chick embryo limb bud cells. Alcian blue staining of 4-day stage 20/21 chick micromass cultures electroporated with plasmids encoding LAP-MMP (panel A), LAP-BMP-7 (panel B) or LAP-TGF-b (panel C); (panel D) Quantification of the alcian blue staining including that of a control pcDNA3 expression vector. (p = 0.005 and 0.001, respectively).

Figure Legend Snippet: Figure 3. LAP fusion proteins are latent until activated by cleavage with MMP. Varying dilutions of supernatant from 293Tcells transiently transfected with (A) LAP-IL-10 (with or without MMP treatment) were added to D36 cells (mast cell line). *P £ 0.05 or (B) LAP-IL-4 (with or without MMP treatment) were added to CT6 cells. The effects of LAP-IL-4 on cell proliferation was measured (n = 3) 72 h later (C) HeLa cells permanently transfected with reporter gene NF-kB-luciferase were treated with IL-1b in the presence of varying concentrations of 293Tsupernatants containing LAP-IL-1ra (SEM values too small to be plotted). (D) Effect of LAP-TGF-b on mink lung fibroblast cells expressing PA-1 promoter driven luciferase. *** p = 0.0001. (E) Supernatants from transiently transfected 293T cells expressing LAP-IGF1 were added to CG4 cells. ** p = 0.001 by paired t-test (F) Proliferation of the megakaryoblastic cell line UT-7 was measured after incubation with supernatants from 293T cells transiently transfected with LAP-EPO. Inhibition of MMP by TAPI-2 prevents background EPO release (left panel) (G) Human fetal fibroblasts cells were stimulated for 24 h with 293T cell-supernatants transiently transfected to express human full-length IL-17A or LAP-IL-17A treated or not with MMP1. * pp = 0.0002, ** p = 0.000001. (H) LAP-TGF-b and LAP BMP-7 promote chondrogenesis in vitro in chick embryo limb bud cells. Alcian blue staining of 4-day stage 20/21 chick micromass cultures electroporated with plasmids encoding LAP-MMP (panel A), LAP-BMP-7 (panel B) or LAP-TGF-b (panel C); (panel D) Quantification of the alcian blue staining including that of a control pcDNA3 expression vector. (p = 0.005 and 0.001, respectively).

Techniques Used: Transfection, Luciferase, Expressing, Incubation, Inhibition, In Vitro, Staining, Control, Plasmid Preparation

Figure 3. LAP fusion proteins are latent until activated by cleavage with MMP (continued). Varying dilutions of supernatant from 293T cells transiently transfected with (A) LAP-IL-10 (with or without MMP treatment) were added to D36 cells (mast cell line). *P £ 0.05 or (B) LAP-IL-4 (with or without MMP treatment) were added to CT6 cells. The effects of LAP-IL-4 on cell proliferation was measured (n = 3) 72 h later (C) HeLa cells permanently transfected with reporter gene NF-kB-luciferase were treated with IL-1b in the presence of varying concentrations of 293Tsupernatants containing LAP-IL-1ra (SEM values too small to be plotted). (D) Effect of LAP-TGF-b on mink lung fibroblast cells expressing PA-1 promoter driven luciferase. *** p = 0.0001. (E) Supernatants from transiently transfected 293Tcells expressing LAP-IGF1 were added to CG4 cells. ** p = 0.001 by paired t-test (F) Proliferation of the megakaryoblastic cell line UT-7 was measured after incubation with supernatants from 293Tcells transiently transfected with LAP-EPO. Inhibition of MMP by TAPI-2 prevents background EPO release (left panel) (G) Human fetal fibroblasts cells were stimulated for 24 h with 293T cell-supernatants transiently transfected to express human full-length IL-17A or LAP- IL-17A treated or not with MMP1. * pp = 0.0002, ** p = 0.000001. (H) LAP-TGF-b and LAP BMP-7 promote chondrogenesis in vitro in chick embryo limb bud cells. Alcian blue staining of 4-day stage 20/21 chick micromass cultures electroporated with plasmids encoding LAP-MMP (panel A), LAP-BMP-7 (panel B) or LAP-TGF-b (panel C); (panel D) Quantification of the alcian blue staining including that of a control pcDNA3 expression vector. (p = 0.005 and 0.001, respectively).

Figure Legend Snippet: Figure 3. LAP fusion proteins are latent until activated by cleavage with MMP (continued). Varying dilutions of supernatant from 293T cells transiently transfected with (A) LAP-IL-10 (with or without MMP treatment) were added to D36 cells (mast cell line). *P £ 0.05 or (B) LAP-IL-4 (with or without MMP treatment) were added to CT6 cells. The effects of LAP-IL-4 on cell proliferation was measured (n = 3) 72 h later (C) HeLa cells permanently transfected with reporter gene NF-kB-luciferase were treated with IL-1b in the presence of varying concentrations of 293Tsupernatants containing LAP-IL-1ra (SEM values too small to be plotted). (D) Effect of LAP-TGF-b on mink lung fibroblast cells expressing PA-1 promoter driven luciferase. *** p = 0.0001. (E) Supernatants from transiently transfected 293Tcells expressing LAP-IGF1 were added to CG4 cells. ** p = 0.001 by paired t-test (F) Proliferation of the megakaryoblastic cell line UT-7 was measured after incubation with supernatants from 293Tcells transiently transfected with LAP-EPO. Inhibition of MMP by TAPI-2 prevents background EPO release (left panel) (G) Human fetal fibroblasts cells were stimulated for 24 h with 293T cell-supernatants transiently transfected to express human full-length IL-17A or LAP- IL-17A treated or not with MMP1. * pp = 0.0002, ** p = 0.000001. (H) LAP-TGF-b and LAP BMP-7 promote chondrogenesis in vitro in chick embryo limb bud cells. Alcian blue staining of 4-day stage 20/21 chick micromass cultures electroporated with plasmids encoding LAP-MMP (panel A), LAP-BMP-7 (panel B) or LAP-TGF-b (panel C); (panel D) Quantification of the alcian blue staining including that of a control pcDNA3 expression vector. (p = 0.005 and 0.001, respectively).

Techniques Used: Transfection, Luciferase, Expressing, Incubation, Inhibition, In Vitro, Staining, Control, Plasmid Preparation

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Journal: Expert opinion on drug delivery

Article Title: Latency can be conferred to a variety of cytokines by fusion with latency-associated peptide from TGF-β.

doi: 10.1517/17425247.2013.839655

Figure Lengend Snippet: Figure 2. LAP fusion proteins are expressed and secreted by 293T cells and can be cleaved with MMP1 to release the free cytokine/ growth factor. Western blotting of (A) recombinant LAP-mmp-BMP-7 detected with anti-BMP-7; (B) recombinant LAP-mmp- IL-10detectedwithanti-IL-10;(C)recombinantLAP-mmp-IL-4detectedwithanti-IL-4;(D)recombinantLAP-mmp-IL-EPOdetectedwith anti-IL-EPO; (F) recombinant LAP-mmp-IL-17 detected with anti-IL-17; (E,G and H) recombinant LAP-mmp-IL-1ra, LAP-mmp-TGF-b and LAP-mmp-IGF1 respectively detected with anti-LAP antibody. All blots indicate via the + or -- sign the presence or absence of MMP1.

Article Snippet: Plates were washed extensively with PBS/Tween before incubation with biotinylated goat antihuman IL-1ra (BAF280; R&D Systems) at a concentration of 200 ng/ml for 1 h at room temperature.

Techniques: Western Blot, Recombinant

Journal: Expert opinion on drug delivery

Article Title: Latency can be conferred to a variety of cytokines by fusion with latency-associated peptide from TGF-β.

doi: 10.1517/17425247.2013.839655

Figure Lengend Snippet: Figure 3. LAP fusion proteins are latent until activated by cleavage with MMP. Varying dilutions of supernatant from 293Tcells transiently transfected with (A) LAP-IL-10 (with or without MMP treatment) were added to D36 cells (mast cell line). *P £ 0.05 or (B) LAP-IL-4 (with or without MMP treatment) were added to CT6 cells. The effects of LAP-IL-4 on cell proliferation was measured (n = 3) 72 h later (C) HeLa cells permanently transfected with reporter gene NF-kB-luciferase were treated with IL-1b in the presence of varying concentrations of 293Tsupernatants containing LAP-IL-1ra (SEM values too small to be plotted). (D) Effect of LAP-TGF-b on mink lung fibroblast cells expressing PA-1 promoter driven luciferase. *** p = 0.0001. (E) Supernatants from transiently transfected 293T cells expressing LAP-IGF1 were added to CG4 cells. ** p = 0.001 by paired t-test (F) Proliferation of the megakaryoblastic cell line UT-7 was measured after incubation with supernatants from 293T cells transiently transfected with LAP-EPO. Inhibition of MMP by TAPI-2 prevents background EPO release (left panel) (G) Human fetal fibroblasts cells were stimulated for 24 h with 293T cell-supernatants transiently transfected to express human full-length IL-17A or LAP-IL-17A treated or not with MMP1. * pp = 0.0002, ** p = 0.000001. (H) LAP-TGF-b and LAP BMP-7 promote chondrogenesis in vitro in chick embryo limb bud cells. Alcian blue staining of 4-day stage 20/21 chick micromass cultures electroporated with plasmids encoding LAP-MMP (panel A), LAP-BMP-7 (panel B) or LAP-TGF-b (panel C); (panel D) Quantification of the alcian blue staining including that of a control pcDNA3 expression vector. (p = 0.005 and 0.001, respectively).

Techniques: Transfection, Luciferase, Expressing, Incubation, Inhibition, In Vitro, Staining, Control, Plasmid Preparation

Journal: Expert opinion on drug delivery

Article Title: Latency can be conferred to a variety of cytokines by fusion with latency-associated peptide from TGF-β.

doi: 10.1517/17425247.2013.839655

Figure Lengend Snippet: Figure 3. LAP fusion proteins are latent until activated by cleavage with MMP (continued). Varying dilutions of supernatant from 293T cells transiently transfected with (A) LAP-IL-10 (with or without MMP treatment) were added to D36 cells (mast cell line). *P £ 0.05 or (B) LAP-IL-4 (with or without MMP treatment) were added to CT6 cells. The effects of LAP-IL-4 on cell proliferation was measured (n = 3) 72 h later (C) HeLa cells permanently transfected with reporter gene NF-kB-luciferase were treated with IL-1b in the presence of varying concentrations of 293Tsupernatants containing LAP-IL-1ra (SEM values too small to be plotted). (D) Effect of LAP-TGF-b on mink lung fibroblast cells expressing PA-1 promoter driven luciferase. *** p = 0.0001. (E) Supernatants from transiently transfected 293Tcells expressing LAP-IGF1 were added to CG4 cells. ** p = 0.001 by paired t-test (F) Proliferation of the megakaryoblastic cell line UT-7 was measured after incubation with supernatants from 293Tcells transiently transfected with LAP-EPO. Inhibition of MMP by TAPI-2 prevents background EPO release (left panel) (G) Human fetal fibroblasts cells were stimulated for 24 h with 293T cell-supernatants transiently transfected to express human full-length IL-17A or LAP- IL-17A treated or not with MMP1. * pp = 0.0002, ** p = 0.000001. (H) LAP-TGF-b and LAP BMP-7 promote chondrogenesis in vitro in chick embryo limb bud cells. Alcian blue staining of 4-day stage 20/21 chick micromass cultures electroporated with plasmids encoding LAP-MMP (panel A), LAP-BMP-7 (panel B) or LAP-TGF-b (panel C); (panel D) Quantification of the alcian blue staining including that of a control pcDNA3 expression vector. (p = 0.005 and 0.001, respectively).

Techniques: Transfection, Luciferase, Expressing, Incubation, Inhibition, In Vitro, Staining, Control, Plasmid Preparation

Figure 1. Time course of mRNA expression of IL-1 cyto- kines and receptors produced by cultured huMCs after ag- gregation of FcεRI. Cells were exposed to anti–NP-IgE over- night, followed by the addition of antigen () to paired cell cultures. Total RNA was isolated at the indicated times and the RPA was performed. A representative result is shown (A). To establish the identity of each protected fragment, the known size and migration distance of the unprotected probe was used to prepare a standard curve. Human control RNA (BD Pharmingen) was used as a positive control and yeast tRNA was used as a negative control. Semiquantification of IL-1ra (B), IL-1RI (C), and IL-1 (D) expression in huMCs before (open bars) or after (filled bars) aggregation of FcεRI was performed by measuring the band density of the relative expression of each mRNA with respect to L32 mRNA after each background was subtracted. IL-1RII and IL-1 mRNAs were below detection levels. For IL-1ra and IL-1 the results were repeated in five separate experiments through 8 h using cells cultured from different donors. These data are pre- sented as means SEM; *P 0.05, **P 0.005, ***P 0.001 compared with 0 time. The 4-h error bar in activated cells in D was too small to diagram.

Journal: American journal of respiratory cell and molecular biology

Article Title: Secretion of interleukin-1 receptor antagonist from human mast cells after immunoglobulin E-mediated activation and after segmental antigen challenge.

doi: 10.1165/ajrcmb.25.6.4541

Figure Lengend Snippet: Figure 1. Time course of mRNA expression of IL-1 cyto- kines and receptors produced by cultured huMCs after ag- gregation of FcεRI. Cells were exposed to anti–NP-IgE over- night, followed by the addition of antigen () to paired cell cultures. Total RNA was isolated at the indicated times and the RPA was performed. A representative result is shown (A). To establish the identity of each protected fragment, the known size and migration distance of the unprotected probe was used to prepare a standard curve. Human control RNA (BD Pharmingen) was used as a positive control and yeast tRNA was used as a negative control. Semiquantification of IL-1ra (B), IL-1RI (C), and IL-1 (D) expression in huMCs before (open bars) or after (filled bars) aggregation of FcεRI was performed by measuring the band density of the relative expression of each mRNA with respect to L32 mRNA after each background was subtracted. IL-1RII and IL-1 mRNAs were below detection levels. For IL-1ra and IL-1 the results were repeated in five separate experiments through 8 h using cells cultured from different donors. These data are pre- sented as means SEM; *P 0.05, **P 0.005, ***P 0.001 compared with 0 time. The 4-h error bar in activated cells in D was too small to diagram.

Article Snippet: The following antibodies were applied for 30 min at previously titrated optimal dilutions: goat antihuman IL-1ra (R&D Systems), AA1 to mast-cell tryptase (Dako Corp., Carpinteria, CA), or goat IgG or mouse IgG 1 control (Dako Corp.).

Techniques: Expressing, Produced, Cell Culture, Isolation, Migration, Control, Positive Control, Negative Control

Figure 4. Inhibition of IL-1–induced IL-8 production by A549 epithelial cells by IL-1ra or mast-cell lysates. A549 cells were in- cubated with IL-1 at a concentration of 50 ng/ml for 16 h in the presence or absence of either rhIL-1ra (A) or huMC lysate (B). Cell-free supernates were collected and assayed for IL-8 by ELISA. The increase was downregulated by (A) increasing con- centrations of standard IL-1ra, and (B) increasing concentrations of huMC lysate.

Journal: American journal of respiratory cell and molecular biology

Article Title: Secretion of interleukin-1 receptor antagonist from human mast cells after immunoglobulin E-mediated activation and after segmental antigen challenge.

doi: 10.1165/ajrcmb.25.6.4541

Figure Lengend Snippet: Figure 4. Inhibition of IL-1–induced IL-8 production by A549 epithelial cells by IL-1ra or mast-cell lysates. A549 cells were in- cubated with IL-1 at a concentration of 50 ng/ml for 16 h in the presence or absence of either rhIL-1ra (A) or huMC lysate (B). Cell-free supernates were collected and assayed for IL-8 by ELISA. The increase was downregulated by (A) increasing con- centrations of standard IL-1ra, and (B) increasing concentrations of huMC lysate.

Techniques: Inhibition, Concentration Assay, Enzyme-linked Immunosorbent Assay

Figure 3. Time course of IL-1ra release from cultured huMCs af- ter FcεRI aggregation. huMCs were incubated with anti–NP-IgE overnight at 37C and washed, and NP-BSA was added to aggre- gate FcεRI (time 0). Plates were then centrifuged at the indicated time points, the supernates removed, and cells lysed. IL-1ra was measured by ELISA in both huMC lysates (open bars) and su- pernates (filled bars) of mast cells (*P 0.05, and **P 0.005 compared with time 0). Data presented are the results of three experiments performed on huMCs cultured from a single donor.

Journal: American journal of respiratory cell and molecular biology

Article Title: Secretion of interleukin-1 receptor antagonist from human mast cells after immunoglobulin E-mediated activation and after segmental antigen challenge.

doi: 10.1165/ajrcmb.25.6.4541

Figure Lengend Snippet: Figure 3. Time course of IL-1ra release from cultured huMCs af- ter FcεRI aggregation. huMCs were incubated with anti–NP-IgE overnight at 37C and washed, and NP-BSA was added to aggre- gate FcεRI (time 0). Plates were then centrifuged at the indicated time points, the supernates removed, and cells lysed. IL-1ra was measured by ELISA in both huMC lysates (open bars) and su- pernates (filled bars) of mast cells (*P 0.05, and **P 0.005 compared with time 0). Data presented are the results of three experiments performed on huMCs cultured from a single donor.

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay

Figure 2. IL-1ra protein in huMCs and in supernates from huMCs after aggregation of FcεRI. (A) After staining of permeabilized cells with a phycoerythrin-conjugated anti–IL-1ra mAb (thick line) or an isotype control mouse IgG1 (thin line), FACS analysis was per- formed to detect the intracellular expression of IL-1ra. (B) huMCs were incubated with anti–NP-IgE overnight at 37C and washed, and NP-BSA was added to aggregate FcεRI. Plates were then centrifuged and the supernates removed. IL-1ra was measured by ELISA in supernates of stimulated (filled bar) and unstimulated (open bar) cells at 20 h (n 4; with cells for each experiment cul- tured from separate donors). (C) Western blot analysis of IL-1ra in huMC supernatants and lysates. ST, rhIL-1ra standard; Lys, concentrated lysate; Supp, concentrated supernate; Lys HMC-1, concentrated HMC-1 lysate. These samples were subjected to electrophoresis on 4 to 12% Bis-Tris gel under reducing condi- tions. After electrophoresis, proteins were blotted and incubated with goat antihuman IL-1ra.

Journal: American journal of respiratory cell and molecular biology

Article Title: Secretion of interleukin-1 receptor antagonist from human mast cells after immunoglobulin E-mediated activation and after segmental antigen challenge.

doi: 10.1165/ajrcmb.25.6.4541

Figure Lengend Snippet: Figure 2. IL-1ra protein in huMCs and in supernates from huMCs after aggregation of FcεRI. (A) After staining of permeabilized cells with a phycoerythrin-conjugated anti–IL-1ra mAb (thick line) or an isotype control mouse IgG1 (thin line), FACS analysis was per- formed to detect the intracellular expression of IL-1ra. (B) huMCs were incubated with anti–NP-IgE overnight at 37C and washed, and NP-BSA was added to aggregate FcεRI. Plates were then centrifuged and the supernates removed. IL-1ra was measured by ELISA in supernates of stimulated (filled bar) and unstimulated (open bar) cells at 20 h (n 4; with cells for each experiment cul- tured from separate donors). (C) Western blot analysis of IL-1ra in huMC supernatants and lysates. ST, rhIL-1ra standard; Lys, concentrated lysate; Supp, concentrated supernate; Lys HMC-1, concentrated HMC-1 lysate. These samples were subjected to electrophoresis on 4 to 12% Bis-Tris gel under reducing condi- tions. After electrophoresis, proteins were blotted and incubated with goat antihuman IL-1ra.

Techniques: Staining, Control, Expressing, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Electrophoresis

Figure 5. IL-1ra in human lung mast cells. (A) Sequential 2-m sections of a surgically resected lung immuno- stained for tryptase (i) and IL-1ra (ii), demonstrating colocalization of IL-1ra to tryptase mast cells (filled arrows). Open arrows indicate IL- 1ra tryptase cells. (B) IL-1ra pro- duction from human lung mast cells (106 cells/ml) stimulated with FcεRI aggregation. Purified human lung– derived mast cells were incubated with anti–NP-IgE overnight at 37C and washed, and NP-BSA was added to aggregate FcεRI. Plates were then centrifuged and the supernates re- moved. IL-1ra was measured by ELISA in supernates of stimulated (filled bar) and unstimulated (open bar) cells at 20 h (n 2).

Journal: American journal of respiratory cell and molecular biology

Article Title: Secretion of interleukin-1 receptor antagonist from human mast cells after immunoglobulin E-mediated activation and after segmental antigen challenge.

doi: 10.1165/ajrcmb.25.6.4541

Figure Lengend Snippet: Figure 5. IL-1ra in human lung mast cells. (A) Sequential 2-m sections of a surgically resected lung immuno- stained for tryptase (i) and IL-1ra (ii), demonstrating colocalization of IL-1ra to tryptase mast cells (filled arrows). Open arrows indicate IL- 1ra tryptase cells. (B) IL-1ra pro- duction from human lung mast cells (106 cells/ml) stimulated with FcεRI aggregation. Purified human lung– derived mast cells were incubated with anti–NP-IgE overnight at 37C and washed, and NP-BSA was added to aggregate FcεRI. Plates were then centrifuged and the supernates re- moved. IL-1ra was measured by ELISA in supernates of stimulated (filled bar) and unstimulated (open bar) cells at 20 h (n 2).

Techniques: Staining, Purification, Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay

Figure 6. IL-1ra levels and isoforms in BALF from seg- mental antigen challenge. Segmental antigen challenge was performed as described in MATERIALS AND METHODS, with (A) before- (Pre) and after- (Post) antigen challenge eosin- ophil number (** indicates a significant difference between eosinophil number in BALF after antigen challenge when compared with before challenge; P 0.005; (B) before- and after-challenge total cell number (* indicates a signifi- cant difference between total cell number in BALF after antigen challenge when compared with before challenge; P 0.05); and (C) IL-1ra (filled bars) and tryptase (open bars) found in cell-free supernates from BALF. Normal subjects (Normal) were 10 patients who were nonatopic and nonasthmatic. There was a significant difference (*P 0.05) between IL-1ra levels in BALF after antigen chal- lenge when compared with before-challenge and normal lev- els. (D) Western blotting of BALF shows the 17-kD form as prominent in BALF with a minimal band at 22 kD. ST, rhIL-1ra 17-kD standard; BAL 1 and 2 are representative samples of after-challenge BALF.

Journal: American journal of respiratory cell and molecular biology

Article Title: Secretion of interleukin-1 receptor antagonist from human mast cells after immunoglobulin E-mediated activation and after segmental antigen challenge.

doi: 10.1165/ajrcmb.25.6.4541

Figure Lengend Snippet: Figure 6. IL-1ra levels and isoforms in BALF from seg- mental antigen challenge. Segmental antigen challenge was performed as described in MATERIALS AND METHODS, with (A) before- (Pre) and after- (Post) antigen challenge eosin- ophil number (** indicates a significant difference between eosinophil number in BALF after antigen challenge when compared with before challenge; P 0.005; (B) before- and after-challenge total cell number (* indicates a signifi- cant difference between total cell number in BALF after antigen challenge when compared with before challenge; P 0.05); and (C) IL-1ra (filled bars) and tryptase (open bars) found in cell-free supernates from BALF. Normal subjects (Normal) were 10 patients who were nonatopic and nonasthmatic. There was a significant difference (*P 0.05) between IL-1ra levels in BALF after antigen chal- lenge when compared with before-challenge and normal lev- els. (D) Western blotting of BALF shows the 17-kD form as prominent in BALF with a minimal band at 22 kD. ST, rhIL-1ra 17-kD standard; BAL 1 and 2 are representative samples of after-challenge BALF.

Techniques: Western Blot

$Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.$

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.

Article Snippet: Blots were developed using specific polyclonal antihuman IL-1ra goat antibody (R&D Systems, Abingdon, UK) at 1/100 dilution in PBS with 1% skimmed milk, biotinylated mouse antigoat secondary antibody (Sigma) at 1/100 dilution and extravidin–phosphatase complex (Sigma) at 1/100 dilution in phosphate-free buffer.

Techniques: Western Blot, Comparison, Control

IL-1-induced IL-6 secretion in OMECs, which were incubated with either RPMI (control), 20 µg/ml of neutralizing human IL-1ra (anti-IL-1ra) or recombinant IL-1α (10 ng/ml) for 24 h. IL-6 immunoreactivity in cell culture supernatants was assayed by ELISA. Exogenous IL-1 enhanced IL-6 secretion by 552%, whereas endogenous IL-1 enhanced IL-6 secretion by 261% after IL-1ra neutralization. The ‘% increase’ represents the mean of (test/control) × 100 calculated for the three experiments, each performed with different cell cultures

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: IL-1-induced IL-6 secretion in OMECs, which were incubated with either RPMI (control), 20 µg/ml of neutralizing human IL-1ra (anti-IL-1ra) or recombinant IL-1α (10 ng/ml) for 24 h. IL-6 immunoreactivity in cell culture supernatants was assayed by ELISA. Exogenous IL-1 enhanced IL-6 secretion by 552%, whereas endogenous IL-1 enhanced IL-6 secretion by 261% after IL-1ra neutralization. The ‘% increase’ represents the mean of (test/control) × 100 calculated for the three experiments, each performed with different cell cultures

Techniques: Incubation, Control, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, Neutralization

Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.

Techniques: Staining, Control, Recombinant

Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Modulation of intracellular IL-1ra (a), IL-1β (b) and IL-1ra : IL-1β ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IL-1α, TNFα, IFNγ, IL-10, TGFβ1, IL-4, IL-6, hydrocortisone (HC) or calcium. The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

Techniques: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Modulation of intracellular IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IFN-γ, IL-10, TGFβ1, IL-4, TNFα, IL-6 or hydrocortisone (HC). The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Modulation of intracellular IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated for 24 h with RPMI 1640 alone as control (C), IFN-γ, IL-10, TGFβ1, IL-4, TNFα, IL-6 or hydrocortisone (HC). The concentration of cytokines in culture lysates was determined by ELISA. The results represent the mean of two experiments, each performed from different cultures. P < 0·05.

Techniques: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Summary of intracellular IL-1 family regulation in OMECs, compared with that in skin keratinocytes

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Summary of intracellular IL-1 family regulation in OMECs, compared with that in skin keratinocytes

Techniques: Derivative Assay

Modulation of secreted IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated with RPMI 1640 alone as control (C), TNFα, IL-6, IFN-γ, IL-10 or TGFβ1. The concentration of cytokines in culture supernatants was determined by ELISA. The results represent two experiments, each performed from different cultures. P < 0·05.

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Modulation of secreted IL-1ra (a), IL-1α (b) and IL-1ra : IL-1α ratio (c) by cytokines in oral mucosal cells. Cells were incubated with RPMI 1640 alone as control (C), TNFα, IL-6, IFN-γ, IL-10 or TGFβ1. The concentration of cytokines in culture supernatants was determined by ELISA. The results represent two experiments, each performed from different cultures. P < 0·05.

Techniques: Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

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